Direct method for estimating the total gastrin cell number in the stomach of rats

Autor: G, Willems, M, Nyst, P, Delincé, J, De Graef
Rok vydání: 1976
Předmět:
Zdroj: Gastroenterology. 71(3)
ISSN: 0016-5085
Popis: An original and simple method for counting the total G cell number in the stomach of small rodents is described. Staining the G cells with the peroxidase-antibody technique and counterstaining the nucleus make it possible to correct the nuclear overestimation. Counting directly the total number of G cells in sections from the entire stomach avoids errors due to tissue retraction and to unprecise delineation of the antro-fundic limit.
Databáze: OpenAIRE