| Autor: |
Poonam, Panjwani, Sridhar, Epari, Arti, Karpate, Hemlata, Shirsat, Preetha, Rajsekharan, Ranjan, Basak, Tanuja, Shet, Roshni, Chinoy, Roy, Chacko, Sampada, Gursale, Nayana, Baraskar, Sudeep, Gupta, Rohini, Hawaldar, Sangeeta, Desai |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
The Indian journal of medical research. 132 |
| ISSN: |
0971-5916 |
| Popis: |
Determination of HER2 status in breast cancer has become important to identify potential candidates for anti-HER2 therapy. In this study we compared fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) for the determination of HER2 status in breast cancer patients referred to a tertiary care referral centre.A total of 200 cases of invasive breast cancer were evaluated for HER2 status using IHC and FISH and results were compared.The IHC 3+ (93.9%) and IHC negative (85.9%) cases showed good concordance with the corresponding FISH results; while 66.6 per cent of IHC 2+ cases showed gene amplification by FISH. In addition, hormone receptor expression and HER2 gene status showed a statistically significant inverse association (P0.05).These findings reaffirm IHC as a prudent first-step to screen tissue samples for HER2 status and to determine suitability for technically demanding FISH test and the dual coloured FISH as a gold standard for determination of HER2/neu status in IHC equivocal cases of breast carcinoma. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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