Determination of the breakpoint of the common alpha-thalassaemia deletion in Filipinos in Hawaii

Autor:	J A, Hunt, L, Lee, T A, Donlon, Y E, Hsia
Rok vydání:	1999
Předmět:	Base Sequence alpha-Thalassemia Molecular Sequence Data Humans Chromosome Breakage Sequence Analysis DNA Polymerase Chain Reaction Sequence Deletion
Zdroj:	British journal of haematology. 104(2)
ISSN:	0007-1048
Popis:	The region of the crossover causing the Filipino type of alpha thalassaemia has been determined by examining similarity between the regions which had been indicated as involved in the crossover points by restriction mapping, using the published alpha-globin region DNA sequence. The crossover point was found in 21 base pairs between two Alu sequences using PCR primers flanking these Alu sequences. A simple PCR multiplex assay has been devised to detect heterozygotes and homozygotes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::0aa069720b4fc22ca665355cdcb8e257 https://pubmed.ncbi.nlm.nih.gov/10050709 Zobrazit plný text záznamu Plný text