[Alteration of transport activity of proton pumps in coleoptile cells during early development stages of maize seedlings]
| Autor: | M F, Shishova, O V, Tankeliun, E L, Rudashevskaia, V V, Emel'ianov, N V, Shakhova, A A, Kirpichnikova |
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| Rok vydání: | 2013 |
| Předmět: |
Vacuolar Proton-Translocating ATPases
Valinomycin Cell Membrane Biological Transport Active Dextrans Proton Pump Inhibitors Zea mays Acridine Orange Substrate Specificity Inorganic Pyrophosphatase Proton-Translocating ATPases Adenosine Triphosphate Seedlings Vacuoles Homeostasis Cotyledon Fluorescein-5-isothiocyanate Fluorescent Dyes |
| Zdroj: | Ontogenez. 43(6) |
| ISSN: | 0475-1450 |
| Popis: | Comparative analysis of the transport activity of proton pumps (plasmalemma H+-ATPase, vacuolar H+-ATPase, and vacuolar H+-pyrophosphatase) in the membrane preparations obtained from coleoptile cells ofetiolated maize seedlings (Zea mays L.) was carried out. The highest level ofvacuolar pyrophosphatase activity was observed during the early development of coleoptile cells under growth intensification through the elongation. The role of ATPase pumps of tonoplast and plasmalemma in the transport of hydrogen ions increases during further development. The plasmalemma activity in this process is higher. When the growth stops, the activity of proton pumps becomes significantly lower. Nevertheless, their substrate specificity and sensitivity to proton pump inhibitors do not change, which can be an evidence of physiological significance of pumps in the maintenance of cell homeostasis. |
| Databáze: | OpenAIRE |
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