| Popis: |
PCR-RFLP was used to investigate the distribution differences of apolipoprotein E (APOE) alleles and genotypes between sporadic Alzheimer disease (AD) patients (n = 160) and healthy control individuals (n = 195) in Chinese Han population. The results showed that the allelic frequencies of APOE epsilon 2, epsilon 3 and epsilon 4 were 0.056, 0.713 and 0.231 in AD group respectively, and 0.082, 0.844 and 0.074 in control group respectively. The frequency of epsilon 4 allele was significantly higher in AD cases than in control subjects and epsilon 4 allele was associated with AD by an odds ratio (OR) of 3.82 (chi 2 = 28.70, P0.001). The probability for APOE epsilon 4-carriers to suffer from AD after 65 years old was 5.38 times of that for APOE epsilon 4 non-carriers (chi 2 = 29.76, P0.001), suggesting that age might affect the interaction between APOE epsilon 4 and AD. In addition, our results showed that the distributions of APOE alleles and genotypes were comparable among mild, moderate and severe dementia patients (P0.05), suggesting that APOE gene polymorphism was not likely to contribute to dementia severity of AD patients. The frequency of APOE epsilon 4 genotype in female patients was higher than that in male patients(43.0% vs. 36.5%) and females carrying APOE epsilon 4 allele had higher OR value than corresponding males (4.3 vs. 3.3), but the differences were not statistically significant (P0.05). As to epsilon 2 allele, its frequency was significantly lower in male subgroup than in female subgroup of AD patients and also than in male subgroup of normal control (P0.05), suggesting that epsilon 2 allele was possibly an AD protective factor in Chinese male population. |
