Asparaginase II of Saccharomyces cerevisiae. Characterization of the ASP3 gene
| Autor: | K W, Kim, J Q, Kamerud, D M, Livingston, R J, Roon |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Base Sequence
Nitrogen Genes Fungal Molecular Sequence Data DNA Recombinant Nucleic Acid Hybridization Saccharomyces cerevisiae Molecular Weight Transformation Genetic Gene Expression Regulation Mutation Escherichia coli Asparaginase Electrophoresis Polyacrylamide Gel Amino Acid Sequence RNA Messenger Cloning Molecular Codon DNA Fungal Plasmids |
| Zdroj: | The Journal of biological chemistry. 263(24) |
| ISSN: | 0021-9258 |
| Popis: | Purified preparations of asparaginase II of Saccharomyces cerevisiae exhibit two protein bands upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Cloning and sequencing of the ASP3 gene, and partial amino acid sequencing as asparaginase II, imply that both bands are encoded by ASP3 but have different N termini. Northern blot analysis using the cloned ASP3 gene as a probe indicates that nitrogen catabolite repression of asparaginase II is achieved by alteration in mRNA levels. Deletion of sequences greater than 600 base pairs upstream from the initiation AUG codon results in an altered response to certain nitrogen sources in strains containing the truncated gene. |
| Databáze: | OpenAIRE |
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