Induction of anti-double stranded DNA antibodies in normal mice by immunization with bacterial DNA

Autor:	G S, Gilkeson, J P, Grudier, D G, Karounos, D S, Pisetsky
Rok vydání:	1989
Předmět:	DNA Bacterial Male Antigens Bacterial Mice Inbred BALB C DNA Antibodies Bacterial Micrococcus Mice Inbred C57BL Kinetics Mice Antibody Specificity Antibodies Antinuclear Escherichia coli Animals Binding Sites Antibody
Zdroj:	Journal of immunology (Baltimore, Md. : 1950). 142(5)
ISSN:	0022-1767
Popis:	Because of recent observations suggesting that bacterial DNA is immunogenic, the induction in normal mice of antibodies to Escherichia coli (EC) dsDNA was investigated. BALB/c and C57BL/6 mice were immunized with dsEC or ds calf thymus (CT) DNA complexed to methylated BSA in adjuvant; antibody responses were measured by ELISA. In both strains, dsEC DNA immunization induced a much higher anti-dsDNA response to dsEC DNA than did dsCT DNA immunization. Neither immunized group showed an appreciable antibody response when tested on dsCT DNA. Anti-dsDNA antibodies were also demonstrated by ELISA using synthetic DNA duplexes as well as a filter binding assay using 3H-labeled dsEC DNA as Ag. These results suggest that bacterial dsDNA is immunogenic and that at least some anti-dsDNA specificities can arise by immunization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::0a2d928b0c5fdbe8882afd8693eac247 https://pubmed.ncbi.nlm.nih.gov/2645362 Zobrazit plný text záznamu