| Autor: |
Mi-Ling, Ma, Gui-Quan, Guan, You-Quan, Li, Ai-Hong, Liu, Qiao-Yun, Ren, Qing-Li, Niu, Hong, Yin, Jian-Xun, Luo |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitologyparasitic diseases. 27(6) |
| ISSN: |
1000-7423 |
| Popis: |
A pair of specific primers was designed based on the reported Bm86 gene of Boophilus microplus,the Bm86 gene was cloned by PCR using the plasmid pMD18-T-Bm86 as templates, and subcloned into the prokaryotic plasmid pGEX-4T-1. The recombined plasmid was transformed into E. coli BL21(DE3) and followed by expression of the protein induced by different concentration of IPTG for different time. SDS-PAGE showed that the recombinant plasmid pGEX-4T-1/Bm86 expressed a fusion protein Bm86-GST (Mr 94 000) after being induced with IPTG. High level expression of Bm86-GST was found at 1 mmol/L IPTG condition a fter incubation for 8 h at 37 degree C, and the expression level of the recombinant Bm86-GST reached up to 29% of total E coli proteins Western-blotting analysis showed that the recombinant Bm86-GST was recognized by the rabbit anti-B. microplus positive serum. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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