| Popis: |
The influence of EDTA on the CO-differential spectra of cytochrome P-420 formed in the rat liver microsomes under the effect of 1 mM CuCl2 in the presence of dithionite has been studied. It was shown that the level of revealed cytochrome P-420 was diminished two-fold following 10 mM EDTA addition if dithionite concentration initially added to the medium was about 2 mM. Cytochrome b5 had no changes. Cytochrome P-420, formed under the effect of desoxycholate, was insensitive to EDTA, but its amount decreased after addition of CuCl2. It is supposed to proceed under chelatation of heme Fe(II). |
