Comparative study of biological activity of three commercial products of Sasa senanensis Rehder leaf extract
| Autor: | Hiroshi, Sakagami, Shoko, Iwamoto, Tomohiko, Matsuta, Kazue, Satoh, Chiyako, Shimada, Taisei, Kanamoto, Shigemi, Terakubo, Hideki, Nakashima, Yurika, Morita, Atsuko, Ohkubo, Tadashi, Tsuda, Katsuyoshi, Sunaga, Madoka, Kitajima, Hiroshi, Oizumi, Takaaki, Oizumi |
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| Rok vydání: | 2012 |
| Předmět: |
Anti-HIV Agents
Cell Survival Ultraviolet Rays T-Lymphocytes Panax Nonprescription Drugs Radiation-Protective Agents Lignin Cell Line Tumor Cytochrome P-450 CYP3A Humans Enzyme Inhibitors Human T-lymphotropic virus 1 Chlorophyllides Plant Extracts Electron Spin Resonance Spectroscopy Free Radical Scavengers Pinus Recombinant Proteins Plant Leaves Drug Combinations Carcinoma Squamous Cell HIV-1 Cytochrome P-450 CYP3A Inhibitors Mouth Neoplasms Sasa |
| Zdroj: | In vivo (Athens, Greece). 26(2) |
| ISSN: | 1791-7549 |
| Popis: | We have previously reported that alkaline extract of Sasa senanensis leaves (SE) has several biological activities characteristic of lignin-carbohydrate complex (LCC). In the present study, we compared the biological activity of three commercially available products of SE (products A, B and C).Cell viability of mock-infected, HIV-infected, UV-irradiated cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Radical intensity was determined by electron spin resonance spectroscopy. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone in human recombinant CYP3A4.Product A is a pure SE that contains Fe(II)-chlorophyllin, whereas products B and C contain Cu(II)-chlorophyllin and less LCC. Product C is supplemented with ginseng and pine (Pinus densiflora) leaf extracts. Product A exhibited 5-fold higher anti-HIV, 4-fold higher anti-UV, 5-fold higher hydroxyl radical-scavenging, and 3-fold lower CYP3A4 inhibitory activities as compared to those of product B, and 5-fold higher, 1.5-fold higher, comparable, and 7-fold lower activities, respectively, as compared to those of product C.The present study demonstrates for the first time the superiority of product A over products B and C, suggesting the beneficial role of LCC and Fe(II)-chlorophyllin. |
| Databáze: | OpenAIRE |
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