| Popis: |
The pulse incorporation of radiolabelled mannitol, glycerol, thymidine, uridine and protein hydrolysate has been studied during the Escherichia coli growth. The incorporation of carbohydrates and predecessors is sharply changed before and after lag-phase, that is due evidently to the partial synchronization of cell population. Three cell divisions have been found: before lag-phase (determined by the curve of optical density), at the end of it and after it termination. Cell division is accompanied by removal of catabolic repression from glycerol utilization, by increase of protein, RNA and DNA synthesis rates. The rates of biopolymer synthesis decrease sharply at the beginning of lag-phase, but DNA synthesis is going on at a rather high level. |
