Chlamydophila psittaci and Chlamydophila pecorum infections in goats and sheep in Egypt
| Autor: | K M, Osman, H A, Ali, J A, ElJakee, H M, Galal |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial Electrophoresis Agar Gel Goat Diseases Sheep Staining and Labeling Chlamydophila Goats Sheep Diseases Chick Embryo Psittacosis Polymerase Chain Reaction Specific Pathogen-Free Organisms Immunoenzyme Techniques Feces Chlamydophila psittaci Fluorescent Antibody Technique Direct Chlorocebus aethiops Animals Egypt Chlamydophila Infections Vero Cells Polymorphism Restriction Fragment Length Bacterial Outer Membrane Proteins |
| Zdroj: | Revue scientifique et technique (International Office of Epizootics). 30(3) |
| ISSN: | 0253-1933 |
| Popis: | The aim of this study was to investigate the epidemiology of chlamydiosis in free-ranging asymptomatic and diarrhoeic sheep and goats in Egypt. Faecal swabs were examined for the presence of Chlamydiae by culture in Vero cells and chick embryos, and staining with Giménez, direct fluorescein-conjugated monoclonal antibodies, and immunoperoxidase. Specific chlamydial DNA was identified by amplification of the omp2 gene. The asymptomatic goats were 50% positive for the presence of the omp2 gene of the family Chlamydiaceae, and all isolates were Chlamydophila psittaci. The percentage of diseased goats in which Chlamydiaceae were identified was 16.2%, and all were positive for Cp. psittaci. Of the asymptomatic sheep, 6.7% were positive for the omp2 gene of the family Chlamydiaceae, and again all were positive for Cp. psittaci. In contrast, 42.9% of the samples that were collected from the diseased sheep were positive for Chlamydiaceae, of which 25.7% were Cp. psittaci and 4.8% Cp. pecorum. |
| Databáze: | OpenAIRE |
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