| Autor: |
D, Кurinnyi, S, Rushkovsky, O, Demchenko, M, Romanenko, T, Liashchenko, M, Pilinska |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Problemy radiatsiinoi medytsyny ta radiobiolohii. 26 |
| ISSN: |
2313-4607 |
| Popis: |
Study the tumor-induced bystander effect of blood cells from chronic lymphocytic leukemia (CLL)patients on non-transformed bystander cells (peripheral blood lymphocytes (PBL) of conditionally healthy individ-uals) and the possibility of its modification after the impact of ionizing radiation.We carried out cocultivation and separate cultivation of blood samples from conditionallyhealthy volunteers and patients with CLL according to our technique. Using the Comet assay, the relative level ofDNA damage was evaluated.A statistically significant increase (р0.001) in the level of DNA damage in PBL culture of conditionallyhealthy individuals after co-cultivation with malignant cells of CLL patients was observed. After irradiation, a drop in the level of cells with a high degree of DNA damage was noted, which was connected with an increase in the frequency of cells that were delayed in division at the S stage of the cell cycle. An increase in apoptotic activity in cultures of bystander cells was observed in all variants of the experiment (р0.001).The influence of irradiated blood cells of patients with CLL results in an enhancement of the tumor-induced bystander effect manifestation in the PBL of conditionally healthy individuals.Meta: doslidyty pukhlynno-indukovanyĭ efekt svidka, vyklykanyĭ vplyvom klityn krovi khvorykh na khronichnyĭlimfoleĭkoz (KhLL), na netransformovani klityny-svidky (limfotsyty peryferychnoï krovi (LPK) umovno zdoro-vykh osib) ta mozhlyvist' ĭogo modyfikatsiï pid vplyvom oprominennia. Materialy i metody. Bulo provedeno spil'no-rozdil'ne kul'tyvuvannia zrazkiv krovi umovno zdorovykh volon-teriv ta khvorykh na KhLL za rozroblenoiu namy metodykoiu z podal'shoiu detektsiieiu vidnosnogo rivnia poshkodzhen-nia DNK za dopomogoiu metodu Commet assay.Rezul'taty. Vstanovleno statystychno znachushche zrostannia (r0,001) rivnia poshkodzhen' DNK v kul'turakh LPKumovno zdorovykh osib, pislia spil'no-rozdil'nogo kul'tyvuvannia z transformovanymy klitynamy khvorykh na KhLL.Pislia oprominennia bulo vidmichene padinnia rivnia klityn z vysokym stupenem poshkodzhen' DNK, shcho vidbulos' zarakhunok zrostannia chastoty klityn, shcho zatrymalys' v podili na S-stadiï klitynnogo tsyklu. Zrostannia apoptychnoïaktyvnosti v kul'turakh klityn-svidkiv bulo vidmicheno v usikh variantakh eksperymentu (r0,001). Vysnovky. Vplyv oprominenykh klityn krovi khvorykh na KhLL pryzvodyt' do posylennia manifestatsiï efektu svidkav LPK umovno zdorovykh osib. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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