High throughput mass spectrometry-based characterisation of

Autor: Aishat, Akere, Qian, Liu, Shibo, Wu, Bingkai, Hou, Min, Yang
Rok vydání: 2018
Zdroj: RSC advances. 8(53)
ISSN: 2046-2069
Popis: In this report, we cloned and characterised four members of group H glycosyltransferases (GTs) by studying their substrate specificities and kinetics. The formation of products and possible glycosylation position was confirmed using MS/MS. The results revealed that 76E1 and 76E5 have broader donor specificity, including UDP-glucose (UDPGlc), UDP-galactose (UDPGal) and UDP
Databáze: OpenAIRE