| Autor: |
Z, Walter, J, Mankiewicz, K, Woźniak, E, Majewska, Z, Baj |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
International journal of occupational medicine and environmental health. 13(3) |
| ISSN: |
1232-1087 |
| Popis: |
The aim of this work was to investigate the possibility of apoptosis in the human peripheral blood lymphocytes after treatment with potassium dichromate (K2Cr2O7), a potential occupational carcinogenic and mutagenic agent. Lymphocytes were stimulated by phytohemagglutinin, cultured for 72 h and incubated with either 0.2 mM or 0.4 mM K2Cr2O7 for the last 24 h or 48 h of culture. The condensation and margination of chromatin with emerging 'half-moon' structure, characteristic of apoptosis were observed. Phosphatidylserine displaced from the inner to outer side of the cellular membrane in 54% of cells after a 48-h incubation with 0.4 mM K2Cr2O7 (annexin-V+/PI-); 39% of these cells were of late apoptotic--secondary necrotic form (annexin-V+/PI+). Following the agarose gel electrophoresis of DNA, a 'ladder pattern' typical of apoptosis, was found. The results of the present study demonstrate that K2Cr2O7 can induce in the human peripheral blood lymphocytes changes similar to apoptotic ones. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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