[Citrate method of extracting antigens from Shigella sonnei. I. Comparison with other methods]

Autor:	N B, Kamzolkina, L M, Iarovaia
Rok vydání:	1981
Předmět:	Endotoxins Antigens Bacterial Epitopes Immunodiffusion Mice Bacterial Proteins Neurotoxins Methods Animals Shigella sonnei Citrates Immunoelectrophoresis
Zdroj:	Zhurnal mikrobiologii, epidemiologii i immunobiologii. (2)
ISSN:	0372-9311
Popis:	The comparative characteristic of extracts obtained from Sh. sonnei by the method of Raynaud and Digeon and neurotoxin obtained by sedimentation with trichloroacetic acid from chloroform autolysates by the method of Mesrobeanu et al. is presented. Both preparations were shown to be similar in their antigenic structure and immunogenicity for mice and guinea-pigs. Nevertheless the extracts obtained by the citrate method contained considerably less protein and endotoxin and had no enterotoxic activity. This allows to recommend the citrate method for extracting protective antigens from Sh. sonnei.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::08dde9e54c081254866e13f9121d91a7 https://pubmed.ncbi.nlm.nih.gov/6166136 Zobrazit plný text záznamu