Effects of pulse methylprednisolone on macrophage chemotactic protein-1 and macrophage inflammatory protein-1alpha in rheumatoid synovium
| Autor: | P K, Wong, C, Cuello, J V, Bertouch, P J, Roberts-Thomson, M J, Ahern, M D, Smith, P P, Youssef |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Macrophages Synovial Membrane Antigens Differentiation Myelomonocytic Cell Count Macrophage Inflammatory Proteins Immunohistochemistry Methylprednisolone Arthritis Rheumatoid Antigens CD Pulse Therapy Drug Injections Intravenous Image Processing Computer-Assisted Humans Female Chemokine CCL4 Chemokine CCL2 Aged Chemokine CCL3 |
| Zdroj: | The Journal of rheumatology. 28(12) |
| ISSN: | 0315-162X |
| Popis: | To determine the effect of pulse methyprednisolone (PMP; 1000 mg) on the expression of monocyte chemoattractant protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha in rheumatoid synovial membrane.Seven patients with rheumatoid arthritis (RA) were studied. Arthroscopically-directed synovial biopsies were taken before and 24 hours after treatment with intravenous PMP. Synovial membranes were stained by immunohistochemical techniques with monoclonal antibodies against MCP-1, MIP-1alpha and CD68 (a macrophage marker). Quantitation of staining was performed by computer-assisted color video image analysis.PMP therapy was associated with a rapid (within 24 hours) and substantial decrease in the expression of MCP-1 and MIP-1alpha expression by a mean of 55% (p = 0.05) and 45% (p = 0.03), respectively, with no effect on CD68 expression in the synovial lining layer. There was no significant change in MCP-1, MIP-1alpha or CD68 expression in the synovial sublining.PMP therapy rapidly reduces MCP-1 and MIP-1alpha levels in the synovial lining layer without a fall in macrophage numbers. It thus appears that the initial effect of PMP is that of reducing macrophage activation. |
| Databáze: | OpenAIRE |
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