Evaluating Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotency Markers
| Autor: | Ricardo Raúl, Cevallos, Md Emon, Hossain, Ruowen, Zhang, Kejin, Hu |
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| Rok vydání: | 2020 |
| Předmět: |
Stage-Specific Embryonic Antigens
SOXB1 Transcription Factors Induced Pluripotent Stem Cells RNA-Binding Proteins Nanog Homeobox Protein Fibroblasts Alkaline Phosphatase Cellular Reprogramming Flow Cytometry Immunophenotyping Antigens Surface Humans Antigens Tumor-Associated Carbohydrate Proteoglycans Octamer Transcription Factor-3 Biomarkers Cells Cultured Transcription Factors |
| Zdroj: | Methods in molecular biology (Clifton, N.J.). 2239 |
| ISSN: | 1940-6029 |
| Popis: | The pluripotency of human induced pluripotent stem cells (HiPSCs) cannot be tested strictly in a similar way as we can do for the mouse ones because of ethical restrictions. One common and initial approach to prove the pluripotency of an established human iPSC line is to demonstrate expression of a set of established surface and intracellular pluripotency markers. This chapter provides procedures of immunocytochemistry of the established HiPSC lines for a set of the signature intracellular pluripotency proteins, OCT4, SOX2, NANOG, and LIN28. We also describe cell phenotyping by flow cytometry for the five established human pluripotency surface markers, SSEA3, SSEA4, TRA-1-60, TRA-1-81, and TRA2-49 (ALP). Numbers of ALP |
| Databáze: | OpenAIRE |
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