| Autor: |
Mikkel R, Holst, Simone S E, Nielsen, Morten S, Nielsen |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2367 |
| ISSN: |
1940-6029 |
| Popis: |
Drug delivery to the brain is a tremendous problem for the academic society and the industry. One solution with a huge potential is to use endocytic receptors as carriers. Here we describe how endocytic activity and subcellular trafficking of a specific receptor in brain endothelial cells can be characterized in three steps. (1) Labeling, endocytosis, and trafficking of a specific receptor at given time points in a pulse-chase experiment. (2) Fixed antibody labeling and co-staining of subcellular markers for image acquisition. (3) Analysis and quantification of co-localization between the receptor and subcellular markers in ImageJ. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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