Withanone from
| Autor: | Acharya, Balkrishna, Subarna, Pokhrel, Hoshiyar, Singh, Monali, Joshi, Vallabh Prakash, Mulay, Swati, Haldar, Anurag, Varshney |
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| Rok vydání: | 2020 |
| Předmět: |
Male
ACE2-RBD complex Static Electricity Molecular Dynamics Simulation Withania Antiviral Agents Structure-Activity Relationship Animals Humans Protein Interaction Domains and Motifs withanone Endoplasmic Reticulum Chaperone BiP Withanolides Withania somnifera Zebrafish Original Research SARS-CoV-2 COVID-19 Virus Internalization COVID-19 Drug Treatment Molecular Docking Simulation Disease Models Animal SARS-CoV-2 S-protein A549 Cells Host-Pathogen Interactions Spike Glycoprotein Coronavirus docking and MD simulation humanized zebrafish model Female ELISA Angiotensin-Converting Enzyme 2 |
| Zdroj: | Drug Design, Development and Therapy |
| ISSN: | 1177-8881 |
| Popis: | Purpose SARS-CoV-2 engages human ACE2 through its spike (S) protein receptor binding domain (RBD) to enter the host cell. Recent computational studies have reported that withanone and withaferin A, phytochemicals found in Withania somnifera, target viral main protease (MPro) and host transmembrane TMPRSS2, and glucose related protein 78 (GRP78), respectively, implicating their potential as viral entry inhibitors. Absence of specific treatment against SARS-CoV-2 infection has encouraged exploration of phytochemicals as potential antivirals. Aim This study aimed at in silico exploration, along with in vitro and in vivo validation of antiviral efficacy of the phytochemical withanone. Methods Through molecular docking, molecular dynamic (MD) simulation and electrostatic energy calculation the plausible biochemical interactions between withanone and the ACE2-RBD complex were investigated. These in silico observations were biochemically validated by ELISA-based assays. Withanone-enriched extract from W. somnifera was tested for its ability to ameliorate clinically relevant pathological features, modelled in humanized zebrafish through SARS-CoV-2 recombinant spike (S) protein induction. Results Withanone bound efficiently at the interacting interface of the ACE2-RBD complex and destabilized it energetically. The electrostatic component of binding free energies of the complex was significantly decreased. The two intrachain salt bridge interactions (K31-E35) and the interchain long-range ion-pair (K31-E484), at the ACE2-RBD interface were completely abolished by withanone, in the 50 ns simulation. In vitro binding assay experimentally validated that withanone efficiently inhibited (IC50=0.33 ng/mL) the interaction between ACE2 and RBD, in a dose-dependent manner. A withanone-enriched extract, without any co-extracted withaferin A, was prepared from W. somnifera leaves. This enriched extract was found to be efficient in ameliorating human-like pathological responses induced in humanized zebrafish by SARS-CoV-2 recombinant spike (S) protein. Conclusion In conclusion, this study provided experimental validation for computational insight into the potential of withanone as a potent inhibitor of SARS-CoV-2 coronavirus entry into the host cells. Video abstract Point your SmartPhone at the code above. If you have a QR code reader the video abstract will appear. Or use: https://youtu.be/6dSVJvU4sGE |
| Databáze: | OpenAIRE |
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