Human bone marrow stroma-dependent cell line MOLP-5 derived from a patient in leukaemic phase of multiple myeloma
| Autor: | Y, Matsuo, H G, Drexler, C, Nishizaki, A, Harashima, S, Fukuda, T, Kozuka, T, Sezaki, K, Orita |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Leukemia Membrane Glycoproteins Interleukin-6 Chromosomes Human Pair 11 Plasma Cells Cell Culture Techniques Interferon-alpha Flow Cytometry ADP-ribosyl Cyclase 1 Antigens Differentiation DNA Fingerprinting Receptors Interleukin-6 Translocation Genetic Cell Line NAD+ Nucleosidase CD28 Antigens Antigens CD Karyotyping Humans CD40 Antigens ADP-ribosyl Cyclase Multiple Myeloma Cell Division Aged |
| Zdroj: | British journal of haematology. 109(1) |
| ISSN: | 0007-1048 |
| Popis: | The novel multiple myeloma (MM) cell line MOLP-5 and its homologous sister cell line B407, a lymphoblastoid cell line (LCL), were established from the peripheral blood of a 71-year-old Japanese patient with Bence-Jones kappa-type multiple myeloma (stage IIIB with hyperammonaemia and hypercalcaemia). The growth of MOLP-5 cells is constitutively dependent on bone marrow stroma (BST) cells; none of the cytokines tested nor the culture supernatant of the bone marrow stroma cells could support the growth of MOLP-5. Wright-Giemsa-stained MOLP-5 cells showed typical plasma cell morphology with abundant cytoplasm and one to three nuclei. The immunoprofile of MOLP-5 corresponds to that seen typically in primary MM cells: positive for cytoplasmic immunoglobulin (Ig) kappa light chain, CD28, CD29, CD38, CD40, CD44, CD49d, CD54, CD56, CD58, CD71, CD138 and PCA-1; the cells were negative for surface Ig and various other B-cell, T-cell and myelomonocyte-associated immunomarkers. Interleukin 6 (IL-6) receptor mRNA was found in the reverse transcriptase polymerase chain reaction (RT-PCR) analysis. IL-6 and IL-10 could induce cellular proliferation in short-term induction experiments. IL-6 or IL-10 production was not detected by specific enzyme-linked immunoabsorbent assay (ELISA). MOLP-5 cells expressed parathyroid hormone-related protein (PTHrP) at the mRNA level. Cytogenetic analysis showed the typical t(11; 14) chromosome abnormality. The novel MOLP-5 cell line together with the B407 B-LCL sister line will be useful model systems in the investigation of the biology of MM. |
| Databáze: | OpenAIRE |
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