Autor: |
A, Burgara-Estrella, M, Montalvo-Corral, A, Bolaños, H, Ramírez-Mendoza, O, Valenzuela, J, Hernández |
Rok vydání: |
2012 |
Předmět: |
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Zdroj: |
Transboundary and emerging diseases. 59(6) |
ISSN: |
1865-1682 |
Popis: |
In this study, the humoral response against porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2), the presence of the virus in semen and serum and the genetic characteristics of the virus detected in 15 boars from a commercial farm were analysed. The results showed that 53% of the boars presented anti-PRRSV antibodies and 100% presented anti-PCV2 antibodies. Porcine reproductive and respiratory syndrome virus was detected in 43% of the boars and 73% were positive to PCV2. The complete ORF5 gene of PRRSV of 14 samples and a fragment of the ORF2 gene of PCV2 of 22 samples were sequenced. Porcine reproductive and respiratory syndrome virus analysis revealed92% identity in viruses from semen and serum of two boars, whereas in the rest of the boars the identity was97.5%. As for PCV2, two boars presented an identity95% in serum and semen and the rest had an identity96%. The results showed that PRRSV- and PCV2-naturally infected boars can be found, and at least two different strains of viruses from semen and serum can be detected. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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