| Autor: |
A V, Kozyr', N M, Luneva, A E, Khlyntseva, I G, Shemiakin, O N, Krasavtseva, A V, Kolesnikov |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (4) |
| ISSN: |
0208-0613 |
| Popis: |
The spread of the New Delhi metallo-beta-lactamase (NDM-1), a plasmid-borne enzyme conferring bacterial resistance to any known beta-lactam antibiotics, represents the global health threat. There is an urgent need to develop the efficient NDM-1 inhibitors of various mode of action thereby necessitating structural studies of the enzyme as well as analysis of the secretion pathway and localization of the protein. The recombinant full-length NDM-1 is produced in E. coli in the inactive form and is mostly accumulated in the inclusion bodies. The secreted recombinant NDM-1 forms are several N-terminally truncated species. The robust expression system capable of high-level production of the full-length NDM-1 and derivatives thereof is required to obtain NDM-1 in the quantities necessary for drug discovery, diagnostics, and research purposes. Therefore, we developed a new system that utilizes antibiotic pressure to select E. coli producing increased quantity of soluble NDM-1 and showed that an increase in the NDM-1 solubility occurs in the bacterial clones producing increased amounts in the chaperones. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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