| Autor: |
Haiming, Huang, Nicolas O, Economopoulos, Bernard A, Liu, Andrea, Uetrecht, Jun, Gu, Nick, Jarvik, Vincent, Nadeem, Tony, Pawson, Jason, Moffat, Shane, Miersch, Sachdev S, Sidhu |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Protein science : a publication of the Protein Society. 24(11) |
| ISSN: |
1469-896X |
| Popis: |
Antibodies are indispensable tools in biochemical research and play an expanding role as therapeutics. While hybridoma technology is the dominant method for antibody production, phage display is an emerging technology. Here, we developed and employed a high-throughput pipeline that enables selection of antibodies against hundreds of antigens in parallel. Binding selections using a phage-displayed synthetic antigen-binding fragment (Fab) library against 110 human SH3 domains yielded hundreds of Fabs targeting 58 antigens. Affinity assays demonstrated that representative Fabs bind tightly and specifically to their targets. Furthermore, we developed an efficient affinity maturation strategy adaptable to high-throughput, which increased affinity dramatically but did not compromise specificity. Finally, we tested Fabs in common cell biology applications and confirmed recognition of the full-length antigen in immunoprecipitation, immunoblotting and immunofluorescence assays. In summary, we have established a rapid and robust high-throughput methodology that can be applied to generate highly functional and renewable antibodies targeting protein domains on a proteome-wide scale. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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