| Autor: |
H T, Rinonce, T H D, Ambarwati, M, Syaebani, M F, Pudjohartono, S, Adevita, P, Ferronika, I, Widodo |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
The Medical journal of Malaysia. 77(Suppl 1) |
| ISSN: |
0300-5283 |
| Popis: |
Urothelial carcinoma (UC) is the most common type of bladder cancer. One of the treatments that are currently being explored for UC involves the use of immune checkpoint inhibitors, especially those targeting PD-1/PD-L1 interaction. This interaction has been previously suggested to aid in the prediction of outcomes. This study was aimed to investigate PD-L1 expression in high grade UC cases in Indonesia, both at mRNA and protein levels, and assess its associated clinicopathological characteristics.The study involved analysis of 51 formalin-fixed paraffin-embedded (FFPE) tissue samples, obtained from patients diagnosed with high grade UC. PD-L1 expression was measured using immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction.PD-L1 IHC staining was found to be positive in five (9.80%) cases. Upregulated expression of PD-L1 mRNA was reported in the patients belonging to older age group (p = 0.049) and mainly involved less invasive cases (p = 0.019), when compared with normoregulated group. Age was positively correlated with PD-L1 expression, as observed in IHC (r = 0.281, p = 0.046). In comparison to this, mitotic index was found to be inversely correlated with PD-L1 mRNA levels (r = -0.369, p = 0.008).IHC staining results showed that PD-L1 expression was lower as compared to previous studies, which might suggest poor response to anti PD-1/PD-L1 agents. The results of the study showed that higher mRNA levels were associated with lower proliferation and less invasive behavior. Thus, the study suggested the potential of PD-L1 mRNA levels to be used as a predictive factor for patient outcomes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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