Stabilization of chimeric BR96-doxorubicin immunoconjugate

Autor:	N P, Barbour, M, Paborji, T C, Alexander, W P, Coppola, J B, Bogardus
Rok vydání:	1995
Předmět:	Chemical Phenomena Chemistry Physical Immunotoxins Temperature Antibodies Monoclonal Enzyme-Linked Immunosorbent Assay Buffers Hydrogen-Ion Concentration Freeze Drying Doxorubicin Freezing Chromatography Gel Spectrophotometry Ultraviolet Chromatography High Pressure Liquid
Zdroj:	Pharmaceutical research. 12(2)
ISSN:	0724-8741
Popis:	Chimeric BR96-doxorubicin conjugate (BR96-DOX) is an immunoconjugate designed to specifically target and kill certain tumor cells. The linker between the chimeric BR96 antibody and DOX is an acid-labile hydrazone group which was designed to undergo lysosomal hydrolysis to release DOX in vivo. Stability studies indicated that acid-catalyzed hydrazone hydrolysis was the major degradation route in vitro. Even under optimal conditions of pH and temperature, the stability of BR96-DOX in solution was not acceptable for long-term storage. Lyophilization of BR96-DOX in the presence of added sugars, such as lactose or sucrose, and subsequent storage of the lyophile under refrigeration significantly improved the stability. Therefore lyophilization appears to be a viable approach for achieving long-term stabilization of BR96-DOX.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::0628272d5c3753d4fdb14969901a91cf https://pubmed.ncbi.nlm.nih.gov/7784336 Zobrazit plný text záznamu Full text from SpringerLink