Testing the Stability of Plasma Protein and Whole Blood RNA in Archived Blood of Loggerhead Sea Turtles

Autor: Kelly, Townsend, Jennifer, Ness, Jennifer, Hoguet, Nicole I, Stacy, Lisa M, Komoroske, Jennifer M, Lynch
Rok vydání: 2020
Předmět:
Zdroj: Biopreservation and biobanking. 18(5)
ISSN: 1947-5543
Popis: Sample storage conditions can affect accuracy and reproducibility of biological measurements. Storing samples rapidly at the lowest available temperatures is considered ideal but is not always feasible when sampling in remote and logistically challenging field conditions, as is often the case with sea turtles. The objective of this study was to examine the stability of plasma proteins and quality of whole blood RNA from loggerhead sea turtle samples collected as part of an eighteen-year-long curated specimen collection. These biological variables are often used to assess sea turtle health; therefore, it is necessary to maintain the integrity of these components during storage. Protein electrophoresis was conducted on heparinized plasma from individual turtles collected in 2018 (
Databáze: OpenAIRE