| Autor: |
H, Crespeau, C, Alliot, J M, Fine, D, Rochu |
| Jazyk: |
francouzština |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie. 313(7) |
| ISSN: |
0764-4469 |
| Popis: |
The diversity of a polyclonal anti-idiotypic response (Ab2) to a murine monoclonal anti-A (Ab1) was investigated after purification of two Ab2 populations. One was eluted from human polyclonal anti-A column and the other from Ab1. Analysis of the Ab specificity, as well as screening of the clonotypic distribution, were achieved after splitting Ab by IEF; this was followed by immunoblotting and probing with various anti-ABH mAb. The first population reacted with almost all the murine anti-ABH mAb, as well as with four human anti-A mAb, and consequently consisted of Ab2 beta. The second was composed of "true" Ab2 directed against Ab1. In the first population internal images mimicked either A Ag, or H Ag, or some epitopes common to both. This study demonstrates the plurality of internal images-bearing Ig molecules, some mimicking completely, and some only partially or even unfaithfully the nominal A determinant. The analysis of this idiotypic cascade proves the existence of a degeneracy of the initial restricted antigenic specificity. The consequences of such a process are discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
