Predominance of neuronal mRNAs in individual Alzheimer's disease senile plaques

Autor:	S D, Ginsberg, P B, Crino, S E, Hemby, J A, Weingarten, V M, Lee, J H, Eberwine, J Q, Trojanowski
Rok vydání:	1999
Předmět:	Male Alzheimer Disease Humans Female Plaque Amyloid RNA Messenger Blotting Northern Immunohistochemistry Polymerase Chain Reaction In Situ Hybridization Aged
Zdroj:	Annals of neurology. 45(2)
ISSN:	0364-5134
Popis:	The sequestration of RNA in Alzheimer's disease (AD) senile plaques (SPs) and the production of intraneuronal amyloid-beta peptides (Abeta) prompted analysis of the mRNA profile in single immunocytochemically identified SPs in sections of AD hippocampus. By using amplified RNA expression profiling, polymerase chain reaction, and in situ hybridization, we assessed the presence and abundance of 51 mRNAs that encode proteins implicated in the pathogenesis of AD. The mRNAs in SPs were compared with those in individual CA1 neurons and the surrounding neuropil of control subjects. The remarkable demonstration here, that neuronal mRNAs predominate in SPs, implies that these mRNAs are nonproteinaceous components of SPs, and, moreover, that mRNAs may interact with Abeta protein and that SPs form at sites where neurons degenerate in the AD brain.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::05ed6e6f59bf345bf24531c6d45d6ac9 https://pubmed.ncbi.nlm.nih.gov/9989619 Zobrazit plný text záznamu Plný text