| Autor: |
Jenna, Beaumont, Mirsaed Miri, Nargesi, Susan, Smith, David, Harte, Erasmus, Smit, James, Ussher, Gary, McAuliffe |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
The New Zealand medical journal. 134(1546) |
| ISSN: |
1175-8716 |
| Popis: |
To assess the sensitivity and potential utility of five RATs and the IDNow, Liat and Oxsed nucleic acid amplification tests (NAATs) in our population.39 retrospective and contrived SARS-CoV-2 positive samples were tested in parallel by standard RT-PCR and RAT. A second group of 44 samples was tested by standard RT-PCR, rapid RT-PCR and two isothermal NAAT assays. Limit of detection was compared at RT-PCR cycle thresholds for all assays.We found that the Cobas Liat RT-PCR had 100% concordance with conventional RT-PCR, whereas the sensitivity of other rapid NAAT assays was less at lower viral loads indicated by Cts30 (p=0.042) and the RATs at Cts25 (p0.001). When applied to New Zealand testing scenarios, IDNow or Oxsed NAAT could miss up to 12% and RATs up to 44.3% of COVID-19 cases compared with the RT-PCR currently used at our laboratory.We found that the POC Cobas Liat, a platform that delivers a sample answer in 20 minutes, demonstrated equivalent performance to standard RT-PCR. However, the RATs and isothermal NAAT assays demonstrated reduced sensitivity, limiting their utility in New Zealand's currently very low prevalence setting. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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