The Main (Glyco) Phospholipid (MPL) of
| Autor: | Hans-Joachim, Freisleben |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
liposomes
bacteriorhodopsin reconstitution light-driven ATP synthesis Differential Thermal Analysis Glycosylation proton permeability Calorimetry Differential Scanning Thermoplasma Archaeal Proteins Review Langmuir-Blodgett monolayer main phospholipid Thermoplasma volcanium Thermoplasma acidophilum lipids (amino acids peptides and proteins) Computer Simulation tetraether lipid Phospholipids black lipid membrane |
| Zdroj: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | The main phospholipid (MPL) of Thermoplasma acidophilum DSM 1728 was isolated, purified and physico-chemically characterized by differential scanning calorimetry (DSC)/differential thermal analysis (DTA) for its thermotropic behavior, alone and in mixtures with other lipids, cholesterol, hydrophobic peptides and pore-forming ionophores. Model membranes from MPL were investigated; black lipid membrane, Langmuir-Blodgett monolayer, and liposomes. Laboratory results were compared to computer simulation. MPL forms stable and resistant liposomes with highly proton-impermeable membrane and mixes at certain degree with common bilayer-forming lipids. Monomeric bacteriorhodopsin and ATP synthase from Micrococcus luteus were co-reconstituted and light-driven ATP synthesis measured. This review reports about almost four decades of research on Thermoplasma membrane and its MPL as well as transfer of this research to Thermoplasma species recently isolated from Indonesian volcanoes. |
| Databáze: | OpenAIRE |
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