Determination of Ro 48-3656 in rat plasma by reversed-phase high-performance liquid chromatography. Comparison of 1.5-microm nonporous silica to 3.5-microm porous silica analytical columns

Autor:	B D, Paasch, Y S, Lin, S, Porter, N B, Modi, T J, Barder
Rok vydání:	1998
Předmět:	Male Quality Control Microchemistry Flunitrazepam Platelet Glycoprotein GPIIb-IIIa Complex Silicon Dioxide Sensitivity and Specificity Rats Rats Sprague-Dawley Heterocyclic Compounds Oximes Animals Prodrugs Chromatography High Pressure Liquid Edetic Acid
Zdroj:	Journal of chromatography. B, Biomedical sciences and applications. 704(1-2)
ISSN:	1387-2273
Popis:	We describe a method for measuring Ro 48-3656 in EDTA rat plasma by neutral pH, reversed-phase high-performance liquid chromatography using a 1.5-microm nonporous silica, C18 analytical column and UV absorbance detection to support pharmacokinetic studies. We also describe a comparison of the 1.5-microm nonporous silica C18 column versus 3.5-microm porous silica C18 columns. The final method using the 1.5-microm nonporous silica column demonstrated good precision (of both quantification and retention time), accuracy and recovery, linearity of dilution and limit of quantification (40 ng/ml Ro 48-3656 using a 20 microl injection). Samples of neat EDTA rat plasma were prepared by ultrafiltration followed by direct injection onto the HPLC column.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::0582d53148e5c12f4b153b946d291bd7 https://pubmed.ncbi.nlm.nih.gov/9518155 Zobrazit plný text záznamu Full Text from ScienceDirect