Effects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma
| Autor: | A, Filip, S, Clichici, A, Muresan, D, Daicoviciu, C, Tatomir, C, Login, S, Dreve, C, Gherman |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Chitosan Photosensitizing Agents Light Anticholesteremic Agents Protoporphyrins Aminolevulinic Acid Combined Modality Therapy Rats Oxidative Stress Carcinosarcoma Matrix Metalloproteinase 9 Photochemotherapy Malondialdehyde Animals Matrix Metalloproteinase 2 Drug Therapy Combination Lipid Peroxidation Rats Wistar |
| Zdroj: | Experimental oncology. 30(3) |
| ISSN: | 1812-9269 |
| Popis: | Porphyrins and new chitosan hydrogels based composites with porphyrins are used as active cytotoxic antitumor agents in photodynamic therapy (PDT).The present study evaluates the effects of photodynamic therapy (PDT) with 5-aminolevulinic acid (5-ALA) and 5-ALA associated with chitosan (CS) using Walker carcinosarcoma in rats as experimental model.The animals were irradiated with red light ( lambda = 685 nm, D = 50 J/cm(2), 15 min) 3 h after i.p. administration of 5-ALA (250 mg/kg b.w.) or a mixture of 5-ALA (250 mg/kg b.w.) and CS (1.5 mg/kg b.w.). The animals were sacrificed at 1, 3, 6, 24 h and 14 days after the treatment. The effects of PDT were investigated by morphological studies, monitoring the 5-ALA induced protoporphyrin IX (Pp IX) level in tumor tissue and serum, MMP 2 and 9 (gelatinases) activity in tumor and malondialdehyde level (MDA), marker of the lipoperoxidation process, in tumor and serum.Zymography revealed an increased activity of MMP 2 in tumors from animals treated with 5-ALA PDT. PDT with 5-ALA induced a higher lipid peroxidation in tumor tissue compared with 5-ALA-CS. CS associated to 5 ALA PDT enhanced the accumulation of PS in tumors inducing earlier necrotic changes. In the same time CS reduced MMP 2 activity.Our results suggest that MMPs activation and oxygen reactive species are involved in PDT effects. |
| Databáze: | OpenAIRE |
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