[HPLC characteristic chromatographic profile of Sarcandra glabra]

Autor: Pei-Ling, Lin, Jian-Wei, Zeng, Chun-Hua, Ding, Yi-Chi, Liang
Rok vydání: 2013
Předmět:
Zdroj: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 38(6)
ISSN: 1001-5302
Popis: To develop the characteristic chromatographic profile of Sarcandra glabra by HPLC for its quality control.The HPLC analysis was performed on an Agilent Zorbax Eclipse XDB-C18 column (4. 6 mm x 250 mm, 5 microm) with column temperature at 40 degree C. The mobile phase was consisted of water containing 0. 5% formic acid and acetonitrile to methanol (1:9) in gradient mode, and the detection wavelength was set at 344 nm.A common mode of the HPLC characteristic chromatographic profile has been establised. There were 20 common peaks , seven of which were identified, and 46 samples from different habitats were classified into five groups based on principal component cluster analysis.The method was time-saving and can represent the chemical information and provide a scientific basis for quality control of S. glabra.
Databáze: OpenAIRE