| Autor: |
Wenqiang, Liu, Guiqin, Liu, Xuling, Qin, Guiying, Wang |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Wei sheng wu xue bao = Acta microbiologica Sinica. 50(4) |
| ISSN: |
0001-6209 |
| Popis: |
Interleukin-18 (IL-18) is a proinflammatory cytokines that plays a key role of immune regulation through activating the Th 1 cell and NK cell secreting interferon-gamma (IFN-gamma). IL-18-binding proteins(IL-18BP) secreted in human and mouse can antagonise the activity of IL-18. The homologue gene of IL-18BP from fowlpox virus genome was predicted and identified of its expressed protein, to use as an antagonist to IL-18 guiding disease.The cIL-18BP gene was isolated from the genome of fowlpox vaccine virus by PCR through a pair of special primers and cloned into vectors pPICZalphaA, then expressed in yeast GS115. The biologic activity of recombinant proteins binding with cIL-18 was measured, inhibiting the activity of cIL-18 enhancing relative cells secreting IFN-gamma was confirmed through ELISA.The cIL-18BP gene was cloned from fowlpox virus and acquired high performance expression in yeast systems induced with methanol identified by SDS-PAGE. Recombinant cIL-18BP purified can bind specifically with recombinant cIL-18 determined by ELISA test. cIL-18BP can antagonise the activity of cIL-18 with determining the IFN-gamma concentration secreting in PBMCs and MSB1 cells stimulated by cIL-18 respectively.The experiment indicates that recombinant cIL-18BP can inhibit the activity of cIL-18 stimulating related immune cells secreting IFN-gamma. Deletion of the cIL-18BP from virus may improve the safety and immunogenicity of fowlpox virus vaccine. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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