Molecular approaches to the differentiation of Actinomyces species

Autor:	C, Jauh-Hsun, T, Vinh, J K, Davies, D, Figdor
Rok vydání:	1999
Předmět:	DNA Bacterial Species Specificity Sequence Analysis RNA RNA Ribosomal 16S Actinomyces Sequence Analysis DNA DNA Probes Oligonucleotide Probes Bacterial Typing Techniques
Zdroj:	Oral microbiology and immunology. 14(4)
ISSN:	0902-0055
Popis:	The definition of the genus Actinomyces relies heavily on traditional methods of taxonomy. This study sought to develop molecular tools for the identification of strains of Actinomyces israelii and Actinomyces gerencseriae. Oligonucleotide probes were designed and one of these successfully differentiated. A. gerencseriae from ten strains of A. israelii and three other Actinomyces species by DNA:DNA hybridization. However, probes based on known 16S rRNA sequences failed to hybridize to all the strains previously identified as A. israelii. Using the PCR technique, a region encoding a portion of the 16S rRNA was amplified from genomic DNA. The results showed that A. israelii can be divided into three different groups based on comparison of the amplified DNA sequences. This information should allow the development of probes that are specific for these newly identified groups of strains within the species A. israelii.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::04763da849c1c788f6ad56b8e4fc0305 https://pubmed.ncbi.nlm.nih.gov/10551170 Zobrazit plný text záznamu Plný text