| Popis: |
Vascular endothelial growth factor C (VEGF-C), a member of the VEGF family, has been reported to promote angiogenesis and tumor cell growth. In this study, we analyzed inhibitory action of a VEGF-C antisense oligoxydeonucleotide (ASODN) on a lung carcinoma cell line A-549 and its invasive ability in vitro.Liposomes, liposome-mediated sense oligoxydeonucleotide (SODN) and ASODN were transfected into A-549 cells, with a blank control group. The expression of VEGF-C was examined by western blotting and invasive ability of cells was detected at four levels.Lower expression of VEGF-C and lower invasive ability to recombinate basal membranes were apparent in the ASOND group (P0.01). However, there were no significant differences among the other three groups (P0.05). In the invasion assay, the number of transmembrane cells was significantly reduced in the ASODN group after 48 hours (58.6±9.2 P0.01), but there was no variation among control, LIP and SOND groups (132.5±15.6, 129.7±16.1, 118.2±12.5, P0.05).VEGF-C ASODN can downregulate the expression of VEGF-C in cell lines and can obviously inhibit invasive ability in vitro. |
