Bca77I: a new type-II restriction endonuclease from Bacillus caldolyticus cutting at the (A/T) increases CCGG(A/T) site

Autor:	T, Schoenfeld, K B, King, M, Schink
Rok vydání:	1992
Předmět:	Base Sequence Molecular Sequence Data Bacillus DNA Deoxyribonucleases Type II Site-Specific Methylation Substrate Specificity
Zdroj:	Gene. 117(1)
ISSN:	0378-1119
Popis:	We describe a new restriction enzyme recognizing a degenerate hexanucleotide sequence and cleaving the 5'-W increases CCGGW site (W = A or T). This enzyme cuts with high efficiency all four permutations of this sequence; ACCGGA (TCCGGT on the opposite strand), ACCGGT and TCCGGA. Methylation of the external cytosine completely blocks restriction while methylation of the internal cytosine only decreases the rate of restriction activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::042f1a4abeed00afeb72be4e56e9cf7b https://pubmed.ncbi.nlm.nih.gov/1644318 Zobrazit plný text záznamu