| Autor: |
Khanit, Sa-Ngiamsuntorn, Ampa, Suksatu, Yongyut, Pewkliang, Piyanoot, Thongsri, Phongthon, Kanjanasirirat, Suwimon, Manopwisedjaroen, Sitthivut, Charoensutthivarakul, Patompon, Wongtrakoongate, Supaporn, Pitiporn, Jarinya, Chaopreecha, Supasek, Kongsomros, Kedchin, Jearawuttanakul, Warawuth, Wannalo, Phisit, Khemawoot, Somchai, Chutipongtanate, Suparerk, Borwornpinyo, Arunee, Thitithanyanont, Suradej, Hongeng |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Journal of Natural Products |
| ISSN: |
1520-6025 |
| Popis: |
The coronaviruses disease 2019 (COVID-19) caused by a novel coronavirus (SARS-CoV-2) has become a major health problem, affecting more than 50 million people with over one million deaths globally. Effective antivirals are still lacking. Here, we optimized a high-content imaging platform and the plaque assay for viral output study using the legitimate model of human lung epithelial cells, Calu-3, to determine the anti-SARS-CoV-2 activity of Andrographis paniculata extract and its major component, andrographolide. SARS-CoV-2 at 25TCID50 was able to reach the maximal infectivity of 95% in Calu-3 cells. Postinfection treatment of A. paniculata and andrographolide in SARS-CoV-2-infected Calu-3 cells significantly inhibited the production of infectious virions with an IC50 of 0.036 μg/mL and 0.034 μM, respectively, as determined by the plaque assay. The cytotoxicity profile developed over the cell line representatives of major organs, including liver (HepG2 and imHC), kidney (HK-2), intestine (Caco-2), lung (Calu-3), and brain (SH-SY5Y), showed a CC50 of >100 μg/mL for A. paniculata extract and 13.2–81.5 μM for andrographolide, respectively, corresponding to a selectivity index of over 380. In conclusion, this study provided experimental evidence in favor of A. paniculata and andrographolide for further development as a monotherapy or in combination with other effective drugs against SARS-CoV-2 infection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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