Quantitative bacterial cultures and cytological examination of bronchoalveolar lavage specimens in dogs
| Autor: | D E, Peeters, B C, McKiernan, R M, Weisiger, D J, Schaeffer, C, Clercx |
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| Rok vydání: | 2000 |
| Předmět: |
Colony Count
Microbial Gram-Positive Bacteria Bronchoalveolar Lavage Sensitivity and Specificity Statistics Nonparametric Dogs Cough Bronchoscopy Gram-Negative Bacteria Animals Dog Diseases Bronchitis Gram-Negative Bacterial Infections Bronchoalveolar Lavage Fluid Respiratory Tract Infections Gram-Positive Bacterial Infections Retrospective Studies |
| Zdroj: | Journal of veterinary internal medicine. 14(5) |
| ISSN: | 0891-6640 |
| Popis: | Cytology and quantitative bacterial cultures of lower respiratory tract secretions are widely used in human medicine to differentiate airway infection from simple bacterial colonization. A retrospective study was conducted to determine the usefulness of quantitative aerobic cultures and Gram stain intracellular bacteria counts from bronchoalveolar lavage (BAL) specimens in dogs in diagnosing lower respiratory tract infection (LRTI) and to determine whether chronic bronchitis is associated with marked bacterial growth in dogs. The threshold determined to define clinically relevant bacterial growth was 1.7 x 10(3) colony-forming units per milliliter of BAL fluid. We used this threshold and found that diagnostic sensitivity and specificity were 86% and 100%, respectively. With a threshold for infection of2 intracellular bacteria observed in any of 50 fields, microscopic examination of Gram stain BAL preparations had a sensitivity of 71% and a specificity of 97% in establishing LRTI. There was a high correlation between bacterial morphology on BAL Gram stain and bacterial cultures. Combining the results of intracellular bacteria counts from the BAL Gram stain with those from the quantitative cultures, the sensitivity in diagnosing LRTI was 87% and the specificity was 97%. BAL quantitative cultures as well as quantitating intracellular bacteria on Gram stain BAL cytology were revealed to be useful in identifying LRTI in dogs. Chronic bronchitis does not appear to be associated with marked bacterial growth in dogs. |
| Databáze: | OpenAIRE |
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