Electron microscopy of the K2 killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast

Autor:	A S, Vadasz, D B, Jagganath, I S, Pretorius, A S, Gupthar
Rok vydání:	2001
Předmět:	Saccharomyces cerevisiae Proteins Wine Intracellular Membranes Saccharomyces cerevisiae Mycotoxins Killer Factors Yeast Culture Media Fungal Proteins Methylene Blue Cell Wall Antibiosis Fermentation Microscopy Electron Scanning Coloring Agents
Zdroj:	Antonie van Leeuwenhoek. 78(2)
ISSN:	0003-6072
Popis:	A mesophilic wine yeast, Saccharomyces cerevisiae CSIR Y217 K-R- was subjected to the K2 killer effect of Saccharomyces cerevisiae T206 K+R+ in a liquid grape medium. The lethal effect of the K2 mycoviral toxin was confirmed by methylene blue staining. Scanning electron microscopy of cells from challenge experiments revealed rippled cell surfaces, accompanied by cracks and pores, while those unaffected by the toxin, as in the control experiments, showed a smooth surface. Transmission electron microscopy revealed that the toxin damaged the cell wall structure and perturbed cytoplasmic membranes to a limited extent.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::037055e9114d7192f11ac71e798e6d9a https://pubmed.ncbi.nlm.nih.gov/11204763 Zobrazit plný text záznamu Full text from SpringerLink