An in vitro priming step increases the expression of numerous epidermal growth and migration mediators in a tissue-engineering construct

Autor: Xiaofeng, Lin, Taehee, Kwak, David, Fiore, Paul J, Thompson, Jane K, Goodrich, Tatyana, Yufit, Aleksandra M, Michalowski, Julie, Deschenes, Polly, Carson, Marta, Otero-Vinas, Vincent, Falanga
Rok vydání: 2014
Předmět:
Zdroj: Journal of tissue engineering and regenerative medicine. 11(3)
ISSN: 1932-7005
Popis: An FDA-approved, prototypic, living, bilayered skin construct (BSC) has been used for non-healing wounds. Using this particular construct as proof of principle, we hypothesized that an in vitro 'priming' step may enhance its repertoire of expression of key mediators and genes. The priming step used here was incubation in Dulbecco's modified Eagle's medium (DMEM) for 24 h at 37°C and 5% CO
Databáze: OpenAIRE