Considerations for Imaging and Analyzing Neural Structures by STED Microscopy

Autor: Martin O, Lenz, Jan, Tønnesen
Rok vydání: 2019
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 1941
ISSN: 1940-6029
Popis: STED microscopy images of live or fixed brain tissue contain a wealth of geometric information about cellular structures down to the scale of individual dendritic spines and axonal structures. To extract such morphological data in a credible way, several considerations regarding image acquisition and analysis must be taken into account. This chapter highlights the parameters of primary importance for acquiring and analyzing STED images and interpreting STED microscopy data.
Databáze: OpenAIRE