| Autor: |
Jun-tao, Li, Heng-wei, Zhang, Xu-hui, Guo, Xian-fu, Sun, Ya-ning, He, Chao-jun, Liu, Shu-de, Cui, Hui, Liu |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Zhonghua yi xue za zhi. 93(2) |
| ISSN: |
0376-2491 |
| Popis: |
To explore the effects and underlying mechanisms of high glucose on in vitro invasiveness of human breast cancer cell line MDA-MB-435.The invasiveness of MDA-MB-435 was determined by Matrigel-coated transwell chambers. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were employed to analyze the cellular expression of matrix metalloproteinase-9/matrix metalloproteinase-2/E-cadherin (MMP-9/MMP-2/E-cadherin) gene/protein.The invasive breast cancer cell numbers of each group (Glu 5.5, 11 and 25 mmol/L) were 50 ± 5, 65 ± 6 and 77 ± 3 respectively. Cellular invasion was dramatically enhanced in the Glu 11 and 25 mmol/L group compared with the 5.5 mmol/L group. The MMP-9/MMP-2 protein expression increased significantly in the Glu 11 and 25 mmol/L groups compared with 5.5 mmol/L group while high glucose (Glu 11 and 25 mmol/L group) down-regulated significantly the E-cadherin mRNA/protein expression.High glucose can promote the in vitro invasiveness of human breast cancer cells through the altered expression of MMP-9/MMP-2/E-cadherin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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