Bcl-2 overexpression results in enhanced capacitative calcium entry and resistance to SKF-96365-induced apoptosis
| Autor: | S S, Williams, J N, French, M, Gilbert, A A, Rangaswami, J, Walleczek, S J, Knox |
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| Rok vydání: | 2000 |
| Předmět: |
Dose-Response Relationship
Drug Drug Resistance Electric Conductivity Imidazoles Apoptosis HL-60 Cells Calcium Channel Blockers Endoplasmic Reticulum Transfection Membrane Potentials Jurkat Cells Proto-Oncogene Proteins c-bcl-2 Electric Impedance Humans Thapsigargin Calcium Calcium Channels Enzyme Inhibitors Ouabain Ion Channel Gating |
| Zdroj: | Cancer research. 60(16) |
| ISSN: | 0008-5472 |
| Popis: | Although there is evidence that changes in cellular ionic concentrations are important early events in apoptosis, the regulation of ion fluxes across the plasma membrane during this process is poorly understood. We report here that Bcl-2 overexpression results in up-regulation of capacitative Ca2+ entry (CCE) and that SKF-96365, an inhibitor of CCE, is a potent inducer of apoptosis. Cells that overexpress Bcl-2 are resistant to SKF-96365-mediated apoptosis and to its inhibition of CCE. Enhanced CCE can be reversed with ouabain, suggesting that Bcl-2-associated plasma membrane hyperpolarization plays a role in up-regulating CCE and may partially explain the antiapoptotic effect of Bcl-2. |
| Databáze: | OpenAIRE |
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