| Autor: |
Roman, Chuprov-Netochin, Elena, Marusich, Yaroslav, Neskorodov, Yana, Mishutkina, Polina, Volynchuk, Yan, Ivanenkov, Alisher, Touraev, Sergey, Leonov, Klaus, Palme |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1795 |
| ISSN: |
1940-6029 |
| Popis: |
Chemical genomics attracts considerable attention by offering crucial tools for plant biology to regulate plant growth and development. However, most chemical screens are time consuming and laborious, and require a high input of resources. Here we describe a broadly applicable method for the ultrarapid high-content phenotypic screening of small chemical compound libraries for new plant growth regulators. The assay is based on determination of pollen tube growth and can be completed in less than 8 h. Using this method, we identified novel inhibitors and modulators of plant growth and showed that compounds selected using a Nicotiana tabacum-based assay were biologically active in other plants, such as Arabidopsis thaliana. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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