| Autor: |
Jonathan, Nylk, Kaley, McCluskey, Sanya, Aggarwal, Javier A, Tello, Kishan, Dholakia |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Biomedical Optics Express |
| ISSN: |
2156-7085 |
| Popis: |
We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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