A rapid qPCR method for genetic sex identification of Salmo salar and Salmo trutta including simultaneous elucidation of interspecies hybrid paternity by high-resolution melt analysis

Autor:	M B, Anglès d'Auriac, H A, Urke, T, Kristensen
Rok vydání:	2013
Předmět:	Genetic Markers Male Sex Determination Analysis Base Sequence Chimera Molecular Sequence Data Animals Female Sequence Analysis DNA Polymerase Chain Reaction Polymorphism Single Nucleotide Sequence Alignment Salmonidae
Zdroj:	Journal of fish biology. 84(6)
ISSN:	1095-8649
Popis:	This study presents an improved duplex quantitative polymerase chain reaction (qPCR) method using the master sex-determining gene sdY as a marker for simultaneous genetic sex identification of salmonids of the Salmo genus and paternity elucidation for Salmo salar × Salmo trutta hybrids. This method will provide a new, simple and economical molecular tool for ecological studies of these species as well as for aquaculture purposes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::0223efc3a6e4e04628e9b2e773ef647f https://pubmed.ncbi.nlm.nih.gov/26890132 Zobrazit plný text záznamu Plný text