Single base substitution in OsCDC48 is responsible for premature senescence and death phenotype in rice
Autor: | Qi-Na, Huang, Yong-Feng, Shi, Xiao-Bo, Zhang, Li-Xin, Song, Bao-Hua, Feng, Hui-Mei, Wang, Xia, Xu, Xiao-Hong, Li, Dan, Guo, Jian-Li, Wu |
---|---|
Rok vydání: | 2015 |
Předmět: |
Chlorophyll
Chloroplasts High‐Impact Article AAA‐ATPase Molecular Sequence Data High‐Impact Articles Oryza sativa Real-Time Polymerase Chain Reaction Plant Roots Fluorescence Quantitative Trait Heritable Gene Expression Regulation Plant Tobacco Amino Acid Sequence Photosynthesis Base Pairing Phylogeny Plant Proteins Cell Nucleus Base Sequence Cell Death Genetic Complementation Test food and beverages Chromosome Mapping Oryza Phenotype OsCDC48 Mutation premature senescence and death Functional Omics and Systems Biology Mesophyll Cells Sequence Alignment Subcellular Fractions |
Zdroj: | Journal of Integrative Plant Biology |
ISSN: | 1744-7909 |
Popis: | A premature senescence and death 128 (psd128) mutant was isolated from an ethyl methane sulfonate‐induced rice IR64 mutant bank. The premature senescence phenotype appeared at the six‐leaf stage and the plant died at the early heading stage. psd128 exhibited impaired chloroplast development with significantly reduced photosynthetic ability, chlorophyll and carotenoid contents, root vigor, soluble protein content and increased malonaldehyde content. Furthermore, the expression of senescence‐related genes was significantly altered in psd128. The mutant trait was controlled by a single recessive nuclear gene. Using map‐based strategy, the mutation Oryza sativa cell division cycle 48 (OsCDC48) was isolated and predicted to encode a putative AAA‐type ATPase with 809 amino‐acid residuals. A single base substitution at position C2347T in psd128 resulted in a premature stop codon. Functional complementation could rescue the mutant phenotype. In addition, RNA interference resulted in the premature senescence and death phenotype. OsCDC48 was expressed constitutively in the root, stem, leaf and panicle. Subcellular analysis indicated that OsCDC48:YFP fusion proteins were located both in the cytoplasm and nucleus. OsCDC48 was highly conserved with more than 90% identity in the protein levels among plant species. Our results indicated that the impaired function of OsCDC48 was responsible for the premature senescence and death phenotype. |
Databáze: | OpenAIRE |
Externí odkaz: |