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External genital warts (EGW) are relatively common sexually transmitted diseases. In the majority of cases, low-risk human papilomaviruses (HPV), such as HPV-6 and HPV-11, are responsible but, high-risk types may also be detected and this has a bearing on vaccines for cervical cancer prevention. In this study the incidence of the high-risk HPV types 16, 18, 33 and 52 in EGWs of females from the southwest of Iran was assessed.Seventy-nine women with EGWs participated in this study. Quantitative real-time PCR with gene specific primers and probes for the E6 gene of HPV-16, 18, 33 and 52, were used for the detection of HPV DNA in the tissue and blood samples.Of the 79 tissue specimens, 13 (16.5%) were HPV positive, only genetic materials of HPV-16 and HPV-18 being detected, twelve patients (15.2%) were positive only for HPV-18 and the coexistence of HPV-16 and HPV-18 was shown in one patient. Only one plasma sample showed evidence of HPV-16 with very low viral load.Our data showed that high-risk HPV types can be found in the tissue specimens of EGW samples obtained from female patients in the Southwest of Iran, with HPV-18 as the most abundant type; however, additional studies with a larger population are required to prove the finding and help to determine the most appropriate type of virus for vaccine design for Iranian women. |
